Record #1 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0008-4026&date=2003& volume=81&issue=6&spage=523&atitle=Elements%20regulating%20AGAMOUS%20expres sion%20are%20conserved%20between%20Arabidopsis%20thaliana,%20Brassica%20nap us,%20and%20Linum%20usitatissimum.&aulast=Pylatuik&auinit=Jeffrey TI- Elements regulating AGAMOUS expression are conserved between Arabidopsis thaliana, Brassica napus, and Linum usitatissimum. AU- Pylatuik, Jeffrey D.; Cross, Rebecca H.; Davis, Arthur R.; Bonham-Smith, Peta C. IN- Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, SK, S7N 5E2, Canada Canada; E-Mail: bonhamp@duke.usask.ca JN- Canadian Journal of Botany ; June 2003; v.81, no.6, p.523-530. JA- CAN J BOT AB- To investigate the functional conservation of cis regulatory elements controlling AGAMOUS (AG) expression, we placed the promoter region of AG from Arabidopsis thaliana into a close relative, Brassica napus, and a distantly related species, Linum usitatissimum, and analyzed the subsequent expression patterns in each species. Spatially, the expression patterns in all three species were marginally similar, in that expression was confined primarily to the reproductive organs and nectarium. Within organs however, tissue-specific expression patterns were not conserved between species. Unlike Arabidopsis, the transgenic AG cis elements did not express in the ovules of B. napus and L. usitatissimum. Temporally, the pattern of AG cis-element expression in B. napus was identical to that of Arabidopsis during early development; however, the AG cis elements did not express at all during early flower development in L. usitatissimum. These results suggest that although regulatory factors controlling the generalized local expression of AG have been conserved between these species, those controlling temporal and tissue-specific expression have not. CO- Molecular Genetics (Biochemistry and Molecular Biophysics); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Reproductive System -- Physiology and Biochemistry (CC16504); Plant Physiology, Biochemistry and Biophysics -- Reproduction (CC51512) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Linaceae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis thaliana (Cruciferae); Brassica napus (Cruciferae) -- transgenic; Linum usitatissimum (Linaceae) -- transgenic; Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- ovule -- reproductive system DE- AGAMOUS promoter; cis regulatory elements DE- Arabidopsis thaliana AGAMOUS gene [Arabidopsis thaliana AG gene] (Cruciferae): temporal expression, tissue-specific expression, transgene DE- species relationship DE- Cruciferae (BC25880); Linaceae (BC26285) DT- Article LA- English SS- 0008-4026 CD- CJBOAW ID- PREV200300432846 SI- 0008-4026(2003)81:6<523:ERAEAC>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #2 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0043-1745&date=2003& volume=51&issue=4&spage=503&atitle=Identification%20of%20genes%20induced%20 in%20emerging%20tillers%20of%20wild%20oat%20(Avena%20fatua)%20using%20Arabi dopsis%20microarrays.&aulast=Horvath&auinit=David TI- Identification of genes induced in emerging tillers of wild oat (Avena fatua) using Arabidopsis microarrays. AU- Horvath, David E.; Schaffer, Robert; Wisman, Ellen IN- Biosciences Research Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Fargo, ND, 58105-5674, USA USA; E-Mail: horvathd@fargo.ars.usda.gov JN- Weed Science ; July-August 2003; v.51, no.4, p.503-508. JA- WEED SCI AB- Arabidopsis complementary DNA (cDNA) microarrays were hybridized with labeled cDNA from mature leaves and emerging tillers of wild oat to determine if they could identify gene expression profiles in distantly related species. More than 23% of the >11,000 cDNAs on the array hybridized to the wild oat probe. Transcription patterns detected by hybridization to the arrays are indicators for physiological processes in the tissues tested. Coordinated expression patterns for these genes in Arabidopsis indicate common signals involved in their regulation. The results demonstrate that probing cDNA-based arrays from well-characterized species can provide valuable insight into the signal transduction processes regulating growth and development of poorly characterized species. CO- Development; Molecular Genetics (Biochemistry and Molecular Biophysics); Pest Assessment Control and Management; Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Developmental Biology -- Embryology-General and Descriptive (CC25502); Plant Physiology, Biochemistry and Biophysics -- Growth, Differentiation (CC51510); Agronomy -- Weed Control (CC52518); Pest Control, General; Pesticides; Herbicides (CC54600) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Gramineae -- Monocotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis (Cruciferae); Avena fatua [wild oat] (Gramineae) -- weed; Angiosperms; Dicots; Monocots; Plants; Spermatophytes; Vascular Plants DE- Arabidopsis complementary DNA microarray analysis -- genetic techniques, laboratory techniques DE- gene identification; gene regulation; growth; tiller emergence DE- Gramineae (BC25305); Cruciferae (BC25880) DT- Article LA- English SS- 0043-1745 CD- WEESA6 ID- PREV200300430245 SI- 0043-1745(2003)51:4<503:IOGIIE>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #3 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0032-0781&date=2003& volume=44&issue=7&spage=750&atitle=The%20rapid%20induction%20of%20glutathio ne%20S-transferases%20AtGSTF2%20and%20AtGSTF6%20by%20avirulent%20Pseudomona s%20syringae%20is%20the%20result%20of%20combined%20salicylic%20acid%20and%2 0ethylene%20signaling.&aulast=Lieberherr&auinit=Damien TI- The rapid induction of glutathione S-transferases AtGSTF2 and AtGSTF6 by avirulent Pseudomonas syringae is the result of combined salicylic acid and ethylene signaling. AU- Lieberherr, Damien; Wagner, Ulrich; Dubuis, Pierre-Henri; Metraux, Jean-Pierre; Mauch, Felix IN- E-Mail: damien@bs.aist-nara.ac.jp; Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, 630-0101, Japan Japan JN- Plant and Cell Physiology ; July 2003; v.44, no.7, p.750-757. JA- PLANT CELL PHYSIOL AB- The expression of two members of the glutathione S-transferase (GST) multigene family was studied in Arabidopsis plants inoculated with an avirulent strain of Pseudomonas syringae pv. tomato (Pst). Accumulation of AtGSTF2 and AtGSTF6 transcripts started 4 and 2 h after inoculation, respectively, and clearly preceded the induction of the pathogenesis-related PR-1 gene. The aim of this work was to find the reason for the faster induction of the two GSTs compared with classical salicylic acid (SA)-regulated PR-proteins. Expression studies in P. s. t.-inoculated SA-signaling mutants NahG and npr1 revealed that induction of both GSTs was SA-dependent and partially NPR1-independent. The induction of AtGSTF2 by P. s. t. was also strongly repressed in the ethylene insensitive etr1 mutant. Both GSTs were induced by low amounts of SA (0.1 mM) and ethylene (0.1 ppm) while PR-1 gene expression was unaffected by ethylene. Interestingly, ethylene was about 50-fold less effective in NahG compared with wild-type plants thus suggesting a potentiation effect of SA on ethylene-induced accumulation of AtGST transcripts. Increased AtGST expression in plants inoculated with P. s. t. correlated with increased production of SA and ethylene. However, the initial phase of AtGSTF6 induction was independent of SA- and ethylene-signaling. The jasmonate (JA)-insensitive mutant jar1 showed normal induction kinetics for both GSTs. Our data support the hypothesis that full expression of the pathogen-induced AtGSTF2 and, to a lesser extent AtGSTF6, is the result of combined SA- and ethylene-signaling and that early AtGSTF6 expression depends on additional unknown signaling mechanisms. CO- Chemical Coordination and Homeostasis; Enzymology (Biochemistry and Molecular Biophysics); Infection; Biochemical Studies -- General (CC10060); Enzymes -- General and Comparative Studies; Coenzymes (CC10802); Physiology, General and Miscellaneous -- General (CC12002); Physiology and Biochemistry of Bacteria (CC31000); Plant Physiology, Biochemistry and Biophysics -- Enzymes (CC51518); Plant Physiology, Biochemistry and Biophysics -- General and Miscellaneous (CC51526) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Pseudomonadaceae -- Gram-Negative Aerobic Rods and Cocci, Eubacteria, Bacteria, Microorganisms; Arabidopsis (Cruciferae) -- NahG mutant, host, npr1 mutant; Pseudomonas syringae (Pseudomonadaceae) -- avirulent, pathovar-tomato; Angiosperms; Bacteria; Dicots; Eubacteria; Microorganisms; Plants; Spermatophytes; Vascular Plants DE- ethylene -- signal; glutathione S-transferase [EC 2.5.1.18] -- rapid induction; salicylic acid -- signal; 74-85-1 -- ETHYLENE; 50812-37-8 -- GLUTATHIONE S-TRANSFERASE; 50812-37-8 -- EC 2.5.1.18; 69-72-7 -- SALICYLIC ACID DE- Arabidopsis PR-1 gene [Arabidopsis pathogenesis-related-1 gene] (Cruciferae): induction; Arabidopsis glutathione S-transferase multigene family (Cruciferae); Arabidopsis thaliana AtGSTF2 gene [Arabidopsis thaliana glutathione S-transferase F2 gene] (Cruciferae); Arabidopsis thaliana AtGSTF6 gene [Arabidopsis thaliana glutathione S-transferase F6 gene] (Cruciferae) DE- signaling mechanism DE- Pseudomonadaceae (1992- ) (BC06508); Cruciferae (BC25880) DT- Article LA- English SS- 0032-0781 CD- PCPHA5 ID- PREV200300429980 SI- 0032-0781(2003)44:7<750:TRIOGS>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #4 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0749-503X&date=2003& volume=20&issue=SUPPLEMENT&spage=S243&atitle=Utilization%20of%20S.%20cerevi siae%20mutants%20defective%20in%20high%20affinity%20copper%20uptake%20to%20 characterize%20copper%20transporters%20from%20plants%20and%20mammals.&aulas t=Puig&auinit=Sergi TI- Utilization of S. cerevisiae mutants defective in high affinity copper uptake to characterize copper transporters from plants and mammals. AU- Puig, Sergi; Sancenon, Vicente; Penarrubia, Lola; Thiele, Dennis J. IN- Dept. of Biological Chemistry, University of Michigan Med. Sch, 1301 Catherine Rd, Ann Arbor, MI, 48109-0606, USA USA; E-Mail: spuig@umich.edu JN- Yeast ; July 2003; v.20, no.Supplement 1, p.S243. JA- YEAST CF- XXIst International Conference on Yeast Genetics and Molecular Biology, July 07-12, 2003, Goeteborg, Sweden CO- Membranes (Cell Biology); Molecular Genetics (Biochemistry and Molecular Biophysics); Transport and Circulation; General Biology -- Symposia, Transactions and Proceedings of Conferences, Congresses, Review Annuals (CC00520); Cytology and Cytochemistry -- Plant (CC02504); Cytology and Cytochemistry -- Animal (CC02506); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Genetics and Cytogenetics -- Animal (CC03506); Biochemical Studies -- Minerals (CC10069); Biophysics -- Membrane Phenomena (CC10508); Physiology, General and Miscellaneous -- General (CC12002); Plant Physiology, Biochemistry and Biophysics -- Translocation, Accumulation (CC51520) TX- Ascomycetes -- Fungi, Plantae; Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Mammalia -- Vertebrata, Chordata, Animalia; Arabidopsis thaliana (Cruciferae) -- model plant; Saccharomyces cerevisiae (Ascomycetes) -- defective mutants; mammal (Mammalia); Angiosperms; Animals; Chordates; Dicots; Fungi; Mammals; Microorganisms; Nonhuman Mammals; Nonhuman Vertebrates; Nonvascular Plants; Plants; Spermatophytes; Vascular Plants; Vertebrates DE- copper -- homeostasis, transport, uptake; 7440-50-8 -- COPPER DE- Arabidopsis thaliana COPT1 gene (Cruciferae): copper transporter; Arabidopsis thaliana COPT2 gene (Cruciferae): copper transporter; Arabidopsis thaliana COPT3 gene (Cruciferae): copper transporter; Arabidopsis thaliana COPT4 gene (Cruciferae): copper transporter; Arabidopsis thaliana COPT5 gene (Cruciferae): copper transporter DE- Ascomycetes (BC15100); Cruciferae (BC25880); Mammalia-Unspecified (BC85700) DT- Conference Publication LA- English SS- 0749-503X CD- YESTE3 ID- PREV200300428799 SI- 0749-503X(2003)20:SUPPLEMENT2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #5 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0016-6731&date=2003& volume=164&issue=2&spage=711&atitle=Analysis%20of%20natural%20allelic%20var iation%20at%20seed%20dormancy%20loci%20of%20Arabidopsis%20thaliana.&aulast= Alonso-Blanco&auinit=Carlos TI- Analysis of natural allelic variation at seed dormancy loci of Arabidopsis thaliana. AU- Alonso-Blanco, Carlos; Bentsink, Leonie; Hanhart, Corrie J.; Blankestijn-De Vries, Hetty; Koornneef, Maarten IN- E-Mail: maarten.koornneef@wur.nl; Laboratory of Genetics, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, Netherlands Netherlands JN- Genetics ; June 2003; v.164, no.2, p.711-729. JA- GENETICS AB- Arabidopsis accessions differ largely in their seed dormancy behavior. To understand the genetic basis of this intraspecific variation we analyzed two accessions: the laboratory strain Landsberg erecta (Ler) with low dormancy and the strong-dormancy accession Cape Verde Islands (Cvi). We used a quantitative trait loci (QTL) mapping approach to identify loci affecting the after-ripening requirement measured as the number of days of seed dry storage required to reach 50% germination. Thus, seven QTL were identified and named delay of germination (DOG) 1-7. To confirm and characterize these loci, we developed 12 near-isogenic lines carrying single and double Cvi introgression fragments in a Ler genetic background. The analysis of these lines for germination in water confirmed four QTL (DOG1, DOG2, DOG3, and DOG6) as showing large additive effects in Ler background. In addition, it was found that DOG1 and DOG3 genetically interact, the strong dormancy determined by DOG1-Cvi alleles depending on DOG3-Ler alleles. These genotypes were further characterized for seed dormancy/germination behavior in five other test conditions, including seed coat removal, gibberellins, and an abscisic acid biosynthesis inhibitor. The role of the Ler/Cvi allelic variation in affecting dormancy is discussed in the context of current knowledge of Arabidopsis germination. CO- Development; Molecular Genetics (Biochemistry and Molecular Biophysics); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Developmental Biology -- Embryology-General and Descriptive (CC25502); Plant Physiology, Biochemistry and Biophysics -- Growth, Differentiation (CC51510) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis thaliana (Cruciferae) -- Cvi, Ler, seed; Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- quantitative trait loci DE- Arabidopsis thaliana DOG1 gene [Arabidopsis thaliana delay of germination 1 gene] (Cruciferae); Arabidopsis thaliana DOG2 gene [Arabidopsis thaliana delay of germination 2 gene] (Cruciferae); Arabidopsis thaliana DOG3 gene [Arabidopsis thaliana delay of germination 3 gene] (Cruciferae); Arabidopsis thaliana DOG4 gene [Arabidopsis thaliana delay of germination 4 gene] (Cruciferae); Arabidopsis thaliana DOG5 gene [Arabidopsis thaliana delay of germination 5 gene] (Cruciferae); Arabidopsis thaliana DOG6 gene [Arabidopsis thaliana delay of germination 6 gene] (Cruciferae); Arabidopsis thaliana DOG7 gene [Arabidopsis thaliana delay of germination 7 gene] (Cruciferae) DE- germination; natural allelic variation; seed dormancy DE- Cruciferae (BC25880) DT- Article LA- English SS- 0016-6731 CD- GENTAE ID- PREV200300425263 SI- 0016-6731(2003)164:2<711:AONAVA>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #6 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0261-4189&date=2003& volume=22&issue=12&spage=3142&atitle=Autoregulation%20of%20FCA%20pre-mRNA%2 0processing%20controls%20Arabidopsis%20flowering%20time.&aulast=Quesada&aui nit=Victor TI- Autoregulation of FCA pre-mRNA processing controls Arabidopsis flowering time. AU- Quesada, Victor; Macknight, Richard; Dean, Caroline; Simpson, Gordon G. IN- E-Mail: gordon.simpson@scri.sari.ac.uk; Gene Expression Programme, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK UK JN- EMBO (European Molecular Biology Organization) Journal ; June 16 2003; v.22, no.12, p.3142-3152. JA- EMBO (EUR MOL BIOL ORGAN) J AB- The timing of the transition to flowering is critical for reproductive success in plants. Arabidopsis FCA encodes an RNA-binding protein that promotes flowering. FCA expression is regulated through alternative processing of its pre-mRNA. We demonstrate here that FCA negatively regulates its own expression by ultimately promoting cleavage and polyadenylation within intron 3. This causes the production of a truncated, inactive transcript at the expense of the full-length FCA mRNA, thus limiting the expression of active FCA protein. We show that this negative autoregulation is under developmental control and requires the FCA WW protein interaction domain. Removal of introns from FCA bypasses the autoregulation, and the resulting increased levels of FCA protein overcomes the repression of flowering normally conferred through the up-regulation of FLC by active FRI alleles. The negative autoregulation of FCA may therefore have evolved to limit FCA activity and hence control flowering time. CO- Biochemistry and Molecular Biophysics; Reproductive System (Reproduction); Biochemical Studies -- General (CC10060); Reproductive System -- Physiology and Biochemistry (CC16504); Plant Physiology, Biochemistry and Biophysics -- Reproduction (CC51512); Plant Physiology, Biochemistry and Biophysics -- Chemical Constituents (CC51522) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis (Cruciferae); Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- flower -- reproductive system DE- FCA; FCA pre-mRNA -- cleavage, polyadenylation, processing DE- Arabidopsis FCA gene (Cruciferae); Arabidopsis FRI gene (Cruciferae) DE- flowering time DE- Cruciferae (BC25880) DT- Article LA- English SS- 0261-4189 CD- EMJODG ID- PREV200300425180 SI- 0261-4189(2003)22:12<3142:AOFPPC>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #7 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0950-1991&date=2003& volume=130&issue=16&spage=3735&atitle=Modes%20of%20intercellular%20transcri ption%20factor%20movement%20in%20the%20Arabidopsis%20apex.&aulast=Wu&auinit =Xuelin TI- Modes of intercellular transcription factor movement in the Arabidopsis apex. AU- Wu, Xuelin; Dinneny, Jose R.; Crawford, Katrina M.; Rhee, Yoon; Citovsky, Vitaly; Zambryski, Patricia C.; Weigel, Detlef IN- E-Mail: weigel@weigelworld.org; Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, 92037, USA USA JN- Development (Cambridge) ; August 2003; v.130, no.16, p.3735-3745. JA- DEVELOPMENT (CAMB) AB- A recent and intriguing discovery in plant biology has been that some transcription factors can move between cells. In Arabidopsis thaliana, the floral identity protein LEAFY has strong non-autonomous effects when expressed in the epidermis, mediated by its movement into underlying tissue layers. By contrast, a structurally unrelated floral identity protein, APETALA1, has only limited non-autonomous effects. Using GFP fusions to monitor protein movement in the shoot apical meristem and in floral primordia of Arabidopsis, we found a strong correlation between cytoplasmic localization of proteins and their ability to move to adjacent cells. The graded distribution of several GFP fusions with their highest levels in the cells where they are produced is compatible with the notion that this movement is driven by diffusion. We also present evidence that protein movement is more restricted laterally within layers than it is from L1 into underlying layers of the Arabidopsis apex. Based on these observations, we propose that intercellular movement of transcription factors can occur in a non-targeted fashion as a result of simple diffusion. This hypothesis raises the possibility that diffusion is the default state for many macromolecules in the Arabidopsis apex, unless they are specifically retained. CO- Biochemistry and Molecular Biophysics; Membranes (Cell Biology); Biophysics -- Membrane Phenomena (CC10508); Biochemical Studies -- General (CC10060); Integumentary System -- Physiology and Biochemistry (CC18504); Plant Physiology, Biochemistry and Biophysics -- Chemical Constituents (CC51522) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis thaliana (Cruciferae); Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- cytoplasm; epidermis -- integumentary system; floral primordia; shoot apical meristem DE- APETALA1 -- floral identity protein, intercellular movement, transcription factor; LEAFY -- floral identity protein, intercellular movement, transcription factor DE- protein trafficking; simple diffusion DE- Cruciferae (BC25880) DT- Article LA- English SS- 0950-1991 CD- DEVPED ID- PREV200300423732 SI- 0950-1991(2003)130:16<3735:MOITFM>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #8 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0012-1606&date=2003& volume=259&issue=2&spage=524&atitle=Cell%20polarity%20in%20Arabidopsis%20tr ichomes.&aulast=Huelskamp&auinit=%20Martin TI- Cell polarity in Arabidopsis trichomes. AU- Huelskamp, Martin IN- Botanical Institute III, University of Cologne, Cologne, Germany Germany JN- Developmental Biology ; July 15 2003; v.259, no.2, p.524-525. JA- DEV BIOL CF- 62nd Annual Meeting of the Society for Developmental Biology held Jointly with the International Society of Developmental Biologists, July 30-August 03, 2003, Boston, MA, USA SP- International Society of Developmental Biologists; Society for Developmental Biology CO- Botany; Development; General Biology -- Symposia, Transactions and Proceedings of Conferences, Congresses, Review Annuals (CC00520); Cytology and Cytochemistry -- Animal (CC02506); Developmental Biology -- Embryology-General and Descriptive (CC25502); Botany, General and Systematic -- Dicotyledones (CC50526); Botany, General and Systematic -- General and Miscellaneous (CC50530); Plant Physiology, Biochemistry and Biophysics -- Growth, Differentiation (CC51510) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis (Cruciferae) -- plant model; Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- Arabidopsis trichomes -- branch formation, cell polarity, cell shape, developmental regulation, epidermal cells DE- Cruciferae (BC25880) DT- Conference Publication LA- English SS- 0012-1606 CD- DEBIAO ID- PREV200300422388 SI- 0012-1606(2003)259:2<524:CPIAT>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #9 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0012-1606&date=2003& volume=259&issue=2&spage=523&atitle=Asymmetric%20divisions%20and%20cell%20s ignaling%20during%20epidermal%20cell%20specification%20in%20Arabidopsis.&au last=Bergmann&auinit=Dominique TI- Asymmetric divisions and cell signaling during epidermal cell specification in Arabidopsis. AU- Bergmann, Dominique; Lukowitz, Wolfgang; Somerville, Chris IN- DPB, Carnegie Institution, Stanford, CA, USA USA JN- Developmental Biology ; July 15 2003; v.259, no.2, p.523. JA- DEV BIOL CF- 62nd Annual Meeting of the Society for Developmental Biology held Jointly with the International Society of Developmental Biologists, July 30-August 03, 2003, Boston, MA, USA SP- International Society of Developmental Biologists; Society for Developmental Biology CO- Botany; Cell Biology; General Biology -- Symposia, Transactions and Proceedings of Conferences, Congresses, Review Annuals (CC00520); Cytology and Cytochemistry -- General (CC02502); Cytology and Cytochemistry -- Plant (CC02504); Cytology and Cytochemistry -- Animal (CC02506); Botany, General and Systematic -- Dicotyledones (CC50526); Botany, General and Systematic -- General and Miscellaneous (CC50530) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis (Cruciferae) -- plant model; Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- Arabidopsis leaf epidermal cells -- asymmetric divisions, cell signaling, pavement cells, specification, stomatal guard cells, trichomes; Arabidopsis stem epidermal cells -- asymmetric divisions, cell signaling, pavement cells, specification, stomatal guard cells, trichomes DE- Cruciferae (BC25880) DT- Conference Publication LA- English SS- 0012-1606 CD- DEBIAO ID- PREV200300422383 SI- 0012-1606(2003)259:2<523:ADACSD>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #10 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0012-1606&date=2003& volume=259&issue=2&spage=523&atitle=Radial%20patterning%20in%20Arabidopsis: %20A%20moving%20target.&aulast=Benfey&auinit=P TI- Radial patterning in Arabidopsis: A moving target. AU- Benfey, P. N.; Gallagher, K.; Paquette, A.; Nakajima, K.; Sena, G. IN- Duke University, Durham, NC, USA USA JN- Developmental Biology ; July 15 2003; v.259, no.2, p.523. JA- DEV BIOL CF- 62nd Annual Meeting of the Society for Developmental Biology held Jointly with the International Society of Developmental Biologists, July 30-August 03, 2003, Boston, MA, USA SP- International Society of Developmental Biologists; Society for Developmental Biology CO- Botany; Development; Molecular Genetics (Biochemistry and Molecular Biophysics); General Biology -- Symposia, Transactions and Proceedings of Conferences, Congresses, Review Annuals (CC00520); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Developmental Biology -- Embryology-General and Descriptive (CC25502); Botany, General and Systematic -- Dicotyledones (CC50526); Botany, General and Systematic -- General and Miscellaneous (CC50530); Plant Physiology, Biochemistry and Biophysics -- Growth, Differentiation (CC51510) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis (Cruciferae) -- embryo, plant model; Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- root meristems -- radial patterning, root development role; shoot meristems -- radial patterning, shoot development role DE- Arabidopsis SCARECROW gene (Cruciferae): mutations, root radial patterning regulator; Arabidopsis SHORT-ROOT gene (Cruciferae): mutations, root radial patterning regulator DE- Cruciferae (BC25880) DT- Conference Publication LA- English SS- 0012-1606 CD- DEBIAO ID- PREV200300422381 SI- 0012-1606(2003)259:2<523:RPIAAM>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #11 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0012-1606&date=2003& volume=259&issue=2&spage=521&atitle=The%20Arabidopsis%20heterochronic%20gen e%20ZIPPY%20is%20an%20ARGONAUTE%20family%20member.&aulast=Hunter&auinit=Chr istine TI- The Arabidopsis heterochronic gene ZIPPY is an ARGONAUTE family member. AU- Hunter, Christine; Wu, Gang; Sun, Hui; Poethig, R. Scott IN- University of Pennsylvania, Philadelphia, PA, USA USA JN- Developmental Biology ; July 15 2003; v.259, no.2, p.521-522. JA- DEV BIOL CF- 62nd Annual Meeting of the Society for Developmental Biology held Jointly with the International Society of Developmental Biologists, July 30-August 03, 2003, Boston, MA, USA SP- International Society of Developmental Biologists; Society for Developmental Biology CO- Botany; Development; Molecular Genetics (Biochemistry and Molecular Biophysics); General Biology -- Symposia, Transactions and Proceedings of Conferences, Congresses, Review Annuals (CC00520); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Developmental Biology -- Embryology-General and Descriptive (CC25502); Botany, General and Systematic -- Dicotyledones (CC50526); Botany, General and Systematic -- General and Miscellaneous (CC50530); Plant Physiology, Biochemistry and Biophysics -- Growth, Differentiation (CC51510) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis (Cruciferae) -- adult phase, development, juvenile phase, plant model, reproductive phase; Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- Arabidopsis heterochronic gene [Arabidopsis ZIPPY gene] (Cruciferae): ARGONAUTE gene family member, premature juvenile-adult phase transition DE- Cruciferae (BC25880) DT- Conference Publication LA- English SS- 0012-1606 CD- DEBIAO ID- PREV200300422376 SI- 0012-1606(2003)259:2<521:TAHGZI>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #12 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=1435-8603&date=2003& volume=5&issue=3&spage=245&atitle=Molecular%20and%20physiological%20charact erisation%20of%20an%20insertion%20mutant%20in%20the%20ARR21%20putative%20re sponse%20regulator%20gene%20from%20Arabidopsis%20thaliana.&aulast=Horak&aui nit=J TI- Molecular and physiological characterisation of an insertion mutant in the ARR21 putative response regulator gene from Arabidopsis thaliana. AU- Horak, J.; Brzobohaty, B.; Lexa, M. IN- Department of Functional Genomics and Proteomics, Faculty of Science, Masaryk University, Kotlarska 2, 61137, Brno, Czech Republic Czech Republic; E-Mail: kubula@sci.muni.cz JN- Plant Biology (Stuttgart) ; May 2003; v.5, no.3, p.245-254. AB- In our search for insertion mutants of Arabidopsis response regulator (ARR) genes, we identified a candidate for an ARR21 dSpm transposon insertion line in the SLAT collection by searching the SINS sequence database. Molecular characterisation of this line revealed that the transposon is integrated as a single copy 1727 bases downstream of the ATG signal, within the third intron of the ARR21 gene. The transposon insertion segregated in a Mendelian fashion from heterozygous plants that were allowed to self-pollinate. RT-PCR-based expression analysis showed that ARR21 transcript predominantly accumulates in siliques. In contrast, the full-length ARR21 transcript was not detectable in the ARR21 transposon insertion line, indicating that it harbours an insertion of dSpm transposon in the ARR21 gene. The ARR21 insertion mutant (arr21-1) was subjected to several physiological tests for a possible insertion-linked phenotype. However, our results revealed that the insertion in the ARR21 gene did not cause any alterations in viability and fertility, flowering time, sensitivity to ethylene, cytokinin or red light. We discuss these results in the light of recent findings about the function of the other members of the response regulator gene family of Arabidopsis. CO- Molecular Genetics (Biochemistry and Molecular Biophysics); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Biochemical Studies -- Nucleic Acids, Purines and Pyrimidines (CC10062) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis thaliana (Cruciferae) -- heterozygous; Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- ARR21 dSpm transposon; Arabidopsis response regulator genes -- insertion mutants DE- Arabidopsis thaliana ARR21 gene (Cruciferae): putative response regulator gene; Arabidopsis thaliana arr21-1 gene (Cruciferae): ARR21 insertion mutant DE- RT-PCR [reverse transcriptase-polymerase chain reaction] -- genetic techniques, laboratory techniques; expression analysis -- genetic techniques, laboratory techniques; molecular characterization -- genetic techniques, laboratory techniques DE- SINS sequence database; SLAT collection; response regulator; signal transduction; transposon insertion segregation; two-component system DE- Cruciferae (BC25880) DT- Article LA- English SS- 1435-8603 ID- PREV200300420332 SI- 1435-8603(2003)5:3<245:MAPCOA>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #13 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0021-9258&date=2003& volume=278&issue=30&spage=28246&atitle=Arabidopsis%20histone%20acetyltransf erase%20AtGCN5%20regulates%20the%20floral%20meristem%20activity%20through%2 0the%20WUSCHEL/AGAMOUS%20pathway.&aulast=Bertrand&auinit=Claire TI- Arabidopsis histone acetyltransferase AtGCN5 regulates the floral meristem activity through the WUSCHEL/AGAMOUS pathway. AU- Bertrand, Claire; Bergounioux, Catherine; Domenichini, Severine; Delarue, Marianne; Zhou, Dao-Xiu IN- E-Mail: zhou@ibp.u-psud.fr; Institut de Biotechnologie des Plantes, CNRS UMR 8618, Universite Paris-sud XI, 91405, Orsay, France France JN- Journal of Biological Chemistry ; July 25 2003; v.278, no.30, p.28246-28251. JA- J BIOL CHEM AB- Histone acetyltransferases, which are able to acetylate histone and non-histone proteins, play important roles in gene regulation. Many histone acetyltransferases are related to yeast Gcn5, a component of two transcription regulatory complexes SAGA and ADA. In this work, by characterizing a mutation in the Arabidopsis GCN5 gene (AtGCN5) we studied the regulatory function of this gene in controlling floral meristem activity. We show that in addition to pleiotropic effects on plant development, this mutation also leads to the production of terminal flowers. The flowers show homeotic transformations of petals into stamens and sepals into filamentous structures and produce ectopic carpels. The phenotypes correlate to an expansion of the expression domains within floral meristems of the key regulatory genes WUSCHEL (WUS) and AGAMOUS (AG). These results suggest that AtGCN5 is required to regulate the floral meristem activity through the WUS/AG pathway. This study brings new elements on the elucidation of specific developmental pathways regulated by AtGCN5 and on the control mechanism of meristem regulatory gene expression. CO- Development; Enzymology (Biochemistry and Molecular Biophysics); Genetics; Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Biochemical Studies -- Proteins, Peptides and Amino Acids (CC10064); Enzymes -- General and Comparative Studies; Coenzymes (CC10802); Developmental Biology -- Embryology-General and Descriptive (CC25502); Plant Physiology, Biochemistry and Biophysics -- Growth, Differentiation (CC51510); Plant Physiology, Biochemistry and Biophysics -- Enzymes (CC51518) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis (Cruciferae); Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- meristem -- activity, floral DE- histone; histone acetyltransferase [EC 2.3.1.48]; 9054-51-7 -- HISTONE ACETYLTRANSFERASE; 9054-51-7 -- EC 2.3.1.48 DE- Arabidopsis AGAMOUS gene (Cruciferae); Arabidopsis GCN5 gene (Cruciferae): mutation; Arabidopsis WUSCHEL gene (Cruciferae) DE- Cruciferae (BC25880) DT- Article LA- English SS- 0021-9258 CD- JBCHA3 ID- PREV200300419116 SI- 0021-9258(2003)278:30<28246:AHAART>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #14 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=1040-4651&date=2003& volume=15&issue=8&spage=1717&atitle=The%20interaction%20of%20two%20homeobox %20genes,%20brevipedicellus%20and%20PENNYWISE,%20regulates%20internode%20pa tterning%20in%20the%20arabidopsis%20inflorescence.&aulast=Smith&auinit=Harl ey TI- The interaction of two homeobox genes, brevipedicellus and PENNYWISE, regulates internode patterning in the arabidopsis inflorescence. AU- Smith, Harley M. S.; Hake, Sarah IN- Department of Plant and Microbial Biology, University of California, Berkeley, CA, 94720, USA USA JN- Plant Cell ; August 2003; v.15, no.8, p.1717-1727. JA- PLANT CELL AB- Plant architecture results from the activity of the shoot apical meristem, which initiates leaves, internodes, and axillary meristems. KNOTTED1-like homeobox (KNOX) genes are expressed in specific patterns in the shoot apical meristem and play important roles in plant architecture. KNOX proteins interact with BEL1-like (BELL) homeodomain proteins and together bind a target sequence with high affinity. We have obtained a mutation in one of the Arabidopsis BELL genes, PENNYWISE (PNY), that appears phenotypically similar to the KNOX mutant brevipedicellus (bp). Both bp and pny have randomly shorter internodes and display a slight increase in the number of axillary branches. The double mutant shows a synergistic phenotype of extremely short internodes interspersed with long internodes and increased branching. PNY is expressed in inflorescence and floral meristems and overlaps with BP in a discrete domain of the inflorescence meristem where we propose the internode is patterned. The physical association of the PNY and BP proteins suggests that they participate in a complex that regulates early patterning events in the inflorescence meristem. CO- Molecular Genetics (Biochemistry and Molecular Biophysics); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis thaliana (Cruciferae); Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- floral meristem; shoot apical meristem [SAM] DE- Arabidopsis thaliana BREVIPEDICELLUS gene (Cruciferae): homeobox gene; Arabidopsis thaliana PENNYWISE gene (Cruciferae): homeobox gene DE- inflorescence; internode patterning DE- Cruciferae (BC25880) DT- Article LA- English SS- 1040-4651 CD- PLCEEW ID- PREV200300410449 SI- 1040-4651(2003)15:8<1717:TIOTHG>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #15 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0012-1606&date=2003& volume=259&issue=2&spage=474&atitle=Promoter%20analyses%20of%20SHORTROOT%20 (SHR)%20and%20SCARECROW%20(SCR).&aulast=Matsumoto&auinit=Noritaka TI- Promoter analyses of SHORTROOT (SHR) and SCARECROW (SCR). AU- Matsumoto, Noritaka; Colinas, Juliette; Benfey, Philip IN- Development, Cell, and Molecular Biology Group, Department of Biology, Duke University, Durham, NC, USA USA JN- Developmental Biology ; July 15 2003; v.259, no.2, p.474. JA- DEV BIOL CF- 62nd Annual Meeting of the Society for Developmental Biology held Jointly with the International Society of Developmental Biologists, July 30-August 03, 2003, Boston, MA, USA SP- International Society of Developmental Biologists; Society for Developmental Biology CO- Botany; Molecular Genetics (Biochemistry and Molecular Biophysics); General Biology -- Symposia, Transactions and Proceedings of Conferences, Congresses, Review Annuals (CC00520); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Botany, General and Systematic -- Dicotyledones (CC50526); Botany, General and Systematic -- General and Miscellaneous (CC50530) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis (Cruciferae) -- plant model; Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- Arabidopsis SCARECROW gene [Arabidopsis SCR gene] (Cruciferae): promoter analysis, root cell division role, root cell specification role; Arabidopsis SHORTROOT gene [Arabidopsis SHR gene] (Cruciferae): promoter analysis, root cell division role, root cell specification role DE- gene promoter analysis -- genetic techniques, laboratory techniques DE- Cruciferae (BC25880) DT- Conference Publication LA- English SS- 0012-1606 CD- DEBIAO ID- PREV200300409418 SI- 0012-1606(2003)259:2<474:PAOS(A>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #16 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=1526-498X&date=2003& volume=59&issue=8&spage=847&atitle=Flufenacet%20herbicide%20treatment%20phe nocopies%20the%20fiddlehead%20mutant%20in%20Arabidopsis%20thaliana.&aulast= Lechelt-Kunze&auinit=Christa TI- Flufenacet herbicide treatment phenocopies the fiddlehead mutant in Arabidopsis thaliana. AU- Lechelt-Kunze, Christa; Meissner, Ruth C.; Drewes, Mark; Tietjen, Klaus IN- E-Mail: Klaus.Tietjen@bayercropscience.com; Research, Target Research, Bayer AG, Bayer CropScience, Building 6240, 40789, Monheim, Germany Germany JN- Pest Management Science ; August 2003; v.59, no.8, p.847-856. AB- In order to study the mode of action of herbicides we conducted a pilot study analysing phenotype and gene expression of flufenacet- and benfuresate-treated Arabidopsis thaliana (L) Heynhoe plants. Treatments with either herbicide caused phenocopies of the known Arabidopsis mutant fiddlehead, displaying fused organs and the typical fiddlehead-like inflorescence. Herbicide treatments of other plant species, including monocots, also gave rise to analogous organ fusions, indicating the presence of the target in a broad range of plants. Furthermore, many other herbicides with a proposed similar mode of action, eg chloroacetanilides, produced comparable fusion phenotypes in plants. The fiddlehead gene encodes a putative very-long-chain fatty acid elongase (VLCFAE), which corroborates earlier biochemical results pointing to the inhibition of VLCFA synthesis as mode of action of flufenacet. Gene expression profiles of herbicide-treated plants using the first 8247 gene Arabidopsis gene array of Affymetrix provided additional clues in support of inhibition of VLCFA synthesis. We discuss fiddlehead-like elongases as plant specific targets for flufenacet and many other herbicides. CO- Genetics; Molecular Genetics (Biochemistry and Molecular Biophysics); Pesticides; Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Biochemical Studies -- General (CC10060); Pest Control, General; Pesticides; Herbicides (CC54600) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Monocotyledones -- Angiospermae, Spermatophyta, Plantae; Plantae; Arabidopsis thaliana (Cruciferae); monocot (Monocotyledones); plant (Plantae); Angiosperms; Dicots; Monocots; Plants; Spermatophytes; Vascular Plants DE- inflorescence DE- benfuresate -- herbicide, mode of action; flufenacet -- herbicide, mode of action; very-long-chain fatty acid elongase [VLCFAE]; 68505-69-1 -- BENFURESATE; 142459-58-3 -- FLUFENACET DE- Arabidopsis thaliana fiddlehead gene (Cruciferae) DE- gene expression profiles; organ fusions; phenocopies; phenotypes DE- Plantae-Unspecified (BC11000); Monocotyledones-Unspecified (BC25202); Cruciferae (BC25880) DT- Article LA- English SS- 1526-498X ID- PREV200300407695 SI- 1526-498X(2003)59:8<847:FHTPTF>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #17 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0027-8424&date=2003& volume=100&issue=12&spage=7383&atitle=Apparent%20homology%20of%20expressed% 20genes%20from%20wood-forming%20tissues%20of%20loblolly%20pine%20(Pinus%20t aeda%20L.)%20with%20Arabidopsis%20thaliana.&aulast=Kirst&auinit=Matias TI- Apparent homology of expressed genes from wood-forming tissues of loblolly pine (Pinus taeda L.) with Arabidopsis thaliana. AU- Kirst, Matias; Johnson, Arthur F.; Baucom, Christie; Ulrich, Erin; Hubbard, Kristy; Staggs, Rod; Paule, Charles; Retzel, Ernest; Whetten, Ross; Sederoff, Ronald IN- E-Mail: ron_sederoff@ncsu.edu; Functional Genomics and Genetics Graduate Program, North Carolina State University, Campus Box 7614, Raleigh, NC, 27695, USA USA JN- Proceedings of the National Academy of Sciences of the United States of America ; June 10 2003; v.100, no.12, p.7383-7388. JA- PROC NATL ACAD SCI U S A AB- Pinus taeda L. (loblolly pine) and Arabidopsis thaliana differ greatly in form, ecological niche, evolutionary history, and genome size. Arabidopsis is a small, herbaceous, annual dicotyledon, whereas pines are large, long-lived, coniferous forest trees. Such diverse plants might be expected to differ in a large number of functional genes. We have obtained and analyzed 59,797 expressed sequence tags (ESTs) from wood-forming tissues of loblolly pine and compared them to the gene sequences inferred from the complete sequence of the Arabidopsis genome. Approximately 50% of pine ESTs have no apparent homologs in Arabidopsis or any other angiosperm in public databases. When evaluated by using contigs containing long, high-quality sequences, we find a higher level of apparent homology between the inferred genes of these two species. For those contigs 1,100 bp or longer, apprxeq90% have an apparent Arabidopsis homolog (E value < 10-10). Pines and Arabidopsis last shared a common ancestor apprxeq300 million years ago. Few genes would be expected to retain high sequence similarity for this time if they did not have essential functions. These observations suggest substantial conservation of gene sequence in seed plants. CO- Molecular Genetics (Biochemistry and Molecular Biophysics); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504) TX- Coniferopsida -- Gymnospermae, Spermatophyta, Plantae; Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis thaliana (Cruciferae); Pinus taeda [loblolly pine] (Coniferopsida); Angiosperms; Dicots; Gymnosperms; Plants; Spermatophytes; Vascular Plants DE- wood-forming tissues DE- Arabidopsis genome sequence; apparent expressed gene homology; expressed sequence tags DE- Coniferopsida (BC25102); Cruciferae (BC25880) DT- Article LA- English SS- 0027-8424 CD- PNASA6 ID- PREV200300403561 SI- 0027-8424(2003)100:12<7383:AHOEGF>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #18 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=1055-3177&date=2003& volume=13&issue=2&spage=170&atitle=Arabidopsis%20bactriana%20belongs%20to%2 0Dielsiocharis%20(Brassicaceae).&aulast=Al-Shehbaz&auinit=Ihsan TI- Arabidopsis bactriana belongs to Dielsiocharis (Brassicaceae). AU- Al-Shehbaz, Ihsan A.; Junussov, Sabir J. IN- Missouri Botanical Garden, P.O. Box 299, St. Louis, MO, 63166-0299, USA USA JN- Novon ; Summer 2003; v.13, no.2, p.170-171. JA- NOVON AB- The Tajikistan endemic Arabidopsis bactriana is transferred to Dielsiocharis, a genus previously recognized as monotypic. Detailed description of A. bactriana is provided, and a key distinguishing the two species of Dielsiocharis is given. CO- Systematics and Taxonomy; General Biology -- Taxonomy, Nomenclature and Terminology (CC00504); Botany, General and Systematic -- Dicotyledones (CC50526) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis bactriana (Cruciferae) -- basionym; Dielsiocharis bactriana (Cruciferae) -- new combination; Dielsiocharis kotschyi (Cruciferae); Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- Tajikistan (Asia, Palearctic region) DE- taxonomic description DE- Cruciferae (BC25880); Cruciferae (BC25880) DT- Article LA- English SS- 1055-3177 CD- NOVOEK ID- PREV200300395492 SI- 1055-3177(2003)13:2<170:ABBTD(>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #19 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0167-4412&date=2003& volume=52&issue=2&spage=273&atitle=Arabidopsis%20RecQsim,%20a%20plant-speci fic%20member%20of%20the%20RecQ%20helicase%20family,%20can%20suppress%20the% 20MMS%20hypersensitivity%20of%20the%20yeast%20sgs1%20mutant.&aulast=Bagheri eh-Najjar&auinit=Mohammad TI- Arabidopsis RecQsim, a plant-specific member of the RecQ helicase family, can suppress the MMS hypersensitivity of the yeast sgs1 mutant. AU- Bagherieh-Najjar, Mohammad B.; de Vries, Onno M. H.; Kroon, Johan T. M.; Wright, Emma L.; Elborough, Kieran M.; Hille, Jacques; Dijkwel, Paul P. IN- E-Mail: p.p.dijkwel@biol.rug.nl; Molecular Biology of Plants, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN, Haren, Netherlands Netherlands JN- Plant Molecular Biology ; May 2003; v.52, no.2, p.273-284. JA- PLANT MOL BIOL AB- The Arabidopsis genome contains seven genes that belong to the RecQ family of ATP-dependent DNA helicases. RecQ members in Saccharomyces cerevisiae (SGS1) and man (WRN, BLM and RecQL4) are involved in DNA recombination, repair and genome stability maintenance, but little is known about the function of their plant counterparts. The Arabidopsis thaliana RecQsim gene is remarkably different from the other RecQ-like genes due to an insertion in its helicase domain. We isolated the AtRecQsim orthologues from rice and rape and established the presence of a similar insertion in their helicase domain, which suggests a plant specific function for the insert. The expression pattern of the AtRecQsim gene was compared with the other Arabidopsis RecQ-like members in different tissues and in response to stress. The transcripts of the AtRecQsim gene were found in all plant organs and its accumulation was higher in roots and seedlings, as compared to the other AtRecQ-like members. In contrast to most AtRecQ-like genes, the examined environmental cues did not have a detectable effect on the accumulation of the AtRecQsim transcripts. The budding yeast sgs1 mutant, which is known'to be hypersensitive to the DNA-damaging drug MMS, was transformed with the AtRecQsim cDNA. The AtRecQsim gene suppressed the MMS hypersensitivity phenotype of the sgs1 cells. We propose that the Arabidopsis RecQsim gene, despite its unusual structure, exhibits an evolutionary conserved function. CO- Molecular Genetics (Biochemistry and Molecular Biophysics); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Genetics and Cytogenetics -- Human (CC03508); Biochemical Studies -- Nucleic Acids, Purines and Pyrimidines (CC10062) TX- Ascomycetes -- Fungi, Plantae; Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis (Cruciferae); Saccharomyces cerevisiae (Ascomycetes); Angiosperms; Dicots; Fungi; Microorganisms; Nonvascular Plants; Plants; Spermatophytes; Vascular Plants DE- DNA -- repair; DNA helicase; methyl methanesulfonate [MMS]; 66-27-3 -- METHYL METHANESULFONATE DE- Arabidopsis RecQ gene family (Cruciferae); Arabidopsis RecQ-like genes (Cruciferae); Arabidopsis thaliana RecQsim gene [AtRecQsim gene] (Cruciferae); Saccharomyces cerevisiae SGS1 gene (Ascomycetes); human BLM gene (Hominidae); human RecQL4 gene (Hominidae); human WRN gene (Hominidae) DE- Ascomycetes (BC15100); Cruciferae (BC25880); Hominidae (BC86215) DT- Article LA- English SS- 0167-4412 CD- PMBIDB ID- PREV200300395459 SI- 0167-4412(2003)52:2<273:ARAPMO>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #20 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0031-949X&date=2003& volume=93&issue=6&spage=S110&atitle=NBS-LRR-encoding%20genes%20in%20plant%2 0genomes.&aulast=Meyers&auinit=%20B.%20C. TI- NBS-LRR-encoding genes in plant genomes. AU- Meyers, B. C. IN- Department of Plant and Soil Science, University of Delaware, Newark, DE, 19711, USA USA JN- Phytopathology ; June 2003; v.93, no.6 Supplement, p.S110. JA- PHYTOPATHOLOGY CF- Annual Meeting of the American Phytopathological Society, August 09-13, 2003, Charlotte, North Carolina, USA SP- American Phytopathological Society AB- More than 30 genes encoding NBS-LRR protein motifs have been cloned from a variety of plant species and demonstrated to confer resistance to specific pathogens. These genes are one of the most prevalent classes in plant genomes, with 148 genes in the Arabidopsis genome and over 500 in rice. In Arabidopsis, approximately two-thirds of the 148 NBS-LRR genes encode a Toll-Interleukin homology (TIR) domain at the N-terminus; the remainder encode a coiled-coil (CC) motif at the N-terminus. In addition to these TIR-NBS-LRR (or TNL) and CC-NBS-LRR (or CNL) proteins, we have identified genes encoding potential adapter proteins lacking the LRR but including the domain combinations TIR-X (TX), TIR-NBS (TN), and CC-NBS (CN). Phylogenetic data and analyses of diverse plant genomes indicate that the CNL family may be more ancient than the TNL family, and suggest a recent diversification of TN- and TNL-encoding genes. Within a single plant genome, a variety of genetic events have occurred during the amplification of these gene families. Detailed studies of the NBS-LRR sequences in Arabidopsis and other plant genomes provide insight into mechanisms of resistance gene function and evolution. CO- Evolution and Adaptation; Infection; Molecular Genetics (Biochemistry and Molecular Biophysics); Pathology; General Biology -- Symposia, Transactions and Proceedings of Conferences, Congresses, Review Annuals (CC00520); Evolution (CC01500); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Pathology, General and Miscellaneous -- General (CC12502); Phytopathology -- General and Miscellaneous (CC54518) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Gramineae -- Monocotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis (Cruciferae); rice (Gramineae); Angiosperms; Dicots; Monocots; Plants; Spermatophytes; Vascular Plants DE- CC-NBS protein [CN protein]; CC-NBS-LRR protein [CNL protein]; CNL family; NBS-LRR protein motifs; TIR-NBS protein [TN protein]; TIR-NBS-LRR protein [TNL protein]; TIR-X protein [TX protein]; TNL family; Toll-interleukin homology domain [TIR domain]; coiled-coil motif [CC motif]; potential adapter proteins DE- Arabidopsis NBS-LRR encoding genes (Cruciferae); Arabidopsis TN-encoding genes (Cruciferae); Arabidopsis TNL-encoding genes (Cruciferae); Arabidopsis resistance gene (Cruciferae): evolution, function, mechanism; rice NBS-LRR encoding genes (Gramineae) DE- cloning -- genetic techniques, laboratory techniques; phylogenetic analysis -- mathematical and computer techniques DE- gene family amplification; pathogen resistance; phytopathology; plant genomics DE- Gramineae (BC25305); Cruciferae (BC25880) DT- Conference Publication LA- English SS- 0031-949X CD- PHYTAJ ID- PREV200300393114 SI- 0031-949X(2003)93:62.0.TX;2- CZ- Copyright 2001 BIOSIS Record #21 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0167-4412&date=2003& volume=52&issue=1&spage=135&atitle=Differential%20activities%20of%20the%20A rabidopsis%20phyB/D/E%20phytochromes%20in%20complementing%20phyB%20mutant%2 0phenotypes.&aulast=Sharrock&auinit=Robert TI- Differential activities of the Arabidopsis phyB/D/E phytochromes in complementing phyB mutant phenotypes. AU- Sharrock, Robert A.; Clack, Ted; Goosey, Lynn IN- Department of Plant Sciences and Plant Pathology, Montana State University, 119 AgBioSciences Building, Bozeman, MT, 59717-3150, USA USA; E-Mail: sharrock@montana.edu JN- Plant Molecular Biology ; May 2003; v.52, no.1, p.135-142. JA- PLANT MOL BIOL AB- The Arabidopsis phyB, phyD, and phyE phytochromes regulate plant developmental and growth responses to continuous red light and to the ratio of red to far-red light. They are also more highly related in sequence to each other and more recently derived evolutionarily than phyA and phyC. In order to directly compare the signaling activities of these three photoreceptor apoproteins, an assay was developed based upon complementation of the phyB-1 null mutant phenotype with transgenes consisting of the PHYB promoter (PB) driving expression of the PHYB, PHYD, or PHYE coding sequences. Expression analysis indicates that the PB-phyB, PB-phyD, and PB-phyE transgenes are transcriptionally and translationally active. However, whereas the PB-phyB transgene complements the phyB-1 red light hypocotyl growth phenotype completely, the PB-phyD and PB-phyE transgenes are only weakly active in restoring seedling growth regulation. Red light fluence curves indicate that this difference is not likely to be due to differences in dark reversion rates. The PB-phyD and PB-phyE transgenes also both partially restore the rosette leaf morphology phenotype of the phyB-1 mutant. However, the PB-phyD gene complements the early flowering phenotype of phyB-1 very strongly whereas PB-phyE does not. These results demonstrate that Arabidopsis phyB-related apoproteins differ significantly in their capacities to signal in various seedling and adult plant phytochrome responses. CO- Chemical Coordination and Homeostasis; Molecular Genetics (Biochemistry and Molecular Biophysics); Radiation Biology; Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Radiation -- General (CC06502); Anatomy and Histology, General and Comparative -- Gross Anatomy (CC11102); Physiology, General and Miscellaneous -- General (CC12002); Morphology, Anatomy and Embryology of Plants (CC51000); Plant Physiology, Biochemistry and Biophysics -- Light and Radiation Effects (CC51516); Plant Physiology, Biochemistry and Biophysics -- General and Miscellaneous (CC51526) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis (Cruciferae); Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- hypocotyl -- growth; leaf -- morphology DE- photoreceptor apoproteins -- signaling activity; phyB phytochrome -- differential activity; phyB-related apoproteins; phyD phytochrome -- differential activity; phyE phytochrome -- differential activity; phytochrome A; phytochrome B; phytochrome C; phytochrome D; phytochrome E; red/far-red photoreceptor DE- Arabidopsis P-B-phyB gene (Cruciferae): transgene; Arabidopsis P-B-phyD gene (Cruciferae): transgene; Arabidopsis P-B-phyE gene (Cruciferae): transgene; Arabidopsis phytochrome gene family (Cruciferae) DE- complementing phyB mutant phenotypes; far-red light; flowering time; photomorphogenesis; red light; signaling DE- Cruciferae (BC25880) DT- Article LA- English SS- 0167-4412 CD- PMBIDB ID- PREV200300386420 SI- 0167-4412(2003)52:1<135:DAOTAP>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #22 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0031-949X&date=2003& volume=93&issue=6&spage=S89&atitle=Molecular%20genetic%20dissection%20of%20 the%20RPP7%20resistance%20pathway.&aulast=Wang&auinit=X-J TI- Molecular genetic dissection of the RPP7 resistance pathway. AU- Wang, X.-J.; Hoff, T.; Cuzick, A.; Holub, E. B.; McDowell, J. M. IN- Virginia Tech, Blacksburg, VA, USA USA JN- Phytopathology ; June 2003; v.93, no.6 Supplement, p.S89. JA- PHYTOPATHOLOGY CF- Annual Meeting of the American Phytopathological Society, August 09-13, 2003, Charlotte, North Carolina, USA SP- American Phytopathological Society AB- The Arabidopsis RPP7 gene activates race-specific resistance to the downy mildew pathogen through a signaling mechanism that does not require accumulation of salicylic acid and is not suppressed by mutations in a variety of defense signal transducers (e.g. pad4-1, ndr1-1, npr1-1, pbs2-1). We have constructed a series of double mutants to test for additive or functionally redundant contributions by known defense signaling components. Most of these combinations display a slightly enhanced level of asexual sporulation, with the ndr1/pad4 combination having the strongest effect. All of the double mutants are capable of inducing the HR, but this response is delayed to varying degrees. These observations suggest that RPP7 activates resistance through multiple signaling pathways that collectively regulate the kinetics of the HR. The RPP7 gene belongs to a cluster of eight highly related CC-NBS-LRR genes on Chr.1. The RPP7 gene is 18.6 Kb long and is comprised of long introns within short 5' and 3' UTRs. The RPP7 transcript is alternatively spliced. CO- Infection; Molecular Genetics (Biochemistry and Molecular Biophysics); Pest Assessment Control and Management; General Biology -- Symposia, Transactions and Proceedings of Conferences, Congresses, Review Annuals (CC00520); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Phytopathology -- Parasitism and Resistance (CC54514); Pest Control, General; Pesticides; Herbicides (CC54600) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Phycomycetes -- Fungi, Plantae; Arabidopsis (Cruciferae); downy mildew (Phycomycetes) -- pathogen; Angiosperms; Dicots; Fungi; Microorganisms; Nonvascular Plants; Plants; Spermatophytes; Vascular Plants DE- Arabidopsis RPP7 gene (Cruciferae): disease resistance; Arabidopsis ndr1-1 gene (Cruciferae): defense signal transducer; Arabidopsis npr1-1 gene (Cruciferae): defense signal transducer; Arabidopsis pad4-1 gene (Cruciferae): defense signal transducer; Arabidopsis pbs2-1 gene (Cruciferae): defense signal transducer DE- hypersensitive response DE- Phycomycetes (BC15900); Cruciferae (BC25880) DT- Conference Publication LA- English SS- 0031-949X CD- PHYTAJ ID- PREV200300383966 SI- 0031-949X(2003)93:62.0.TX;2- CZ- Copyright 2001 BIOSIS Record #23 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0950-1991&date=2003& volume=130&issue=14&spage=3283&atitle=Mutations%20in%20Arabidopsis%20conden sin%20genes%20disrupt%20embryogenesis,%20meristem%20organization%20and%20se gregation%20of%20homologous%20chromosomes%20during%20meiosis.&aulast=Siddiq ui&auinit=Najeeb TI- Mutations in Arabidopsis condensin genes disrupt embryogenesis, meristem organization and segregation of homologous chromosomes during meiosis. AU- Siddiqui, Najeeb U.; Stronghill, Patricia E.; Dengler, Ronald E.; Hasenkampf, Clare A.; Riggs, C. Daniel IN- Department of Botany, Division of Life Sciences, University of Toronto, 1265 Military Trail, West Hill, Ontario, M1C 1A4, Canada Canada; E-Mail: riggs@utsc.utoronto.ca JN- Development (Cambridge) ; July 2003; v.130, no.14, p.3283-3295. JA- DEVELOPMENT (CAMB) AB- Proper chromatin condensation and sister chromatid resolution are essential for the maintenance of chromosomal integrity during cell division, and is in part mediated by a conserved multisubunit apparatus termed the condensin complex. The core subunits of the complex are members of the SMC2 (Structural Maintenance of Chromosomes) and SMC4 gene families. We have cloned an Arabidopsis gene, AtCAP-E1, which is a functional ortholog of the yeast SMC2 gene. A second, highly homologous SMC2 gene, AtCAPE-2, was identified by the Arabidopsis genome project. SMC2 gene expression in Arabidopsis was correlated with the mitotic activity of tissues, with high level expression observed in meristematic cells. The two genes are differentially expressed with AtCAP-E1 accounting for more than 85% of the total SMC2 transcript pool. The titan3 mutant is the result of a T-DNA insertion into AtCAP-E1, but other than subtle endosperm defects, titan3 is viable and fecund. We identified a T-DNA insertion mutant of AtCAP-E2, which showed no obvious mutant phenotype, indicating that the two genes are functionally redundant. Genetic crosses were employed to examine the consequences of reduced SMC2 levels. Both male and female gametogenesis were compromised in double mutant spores. Embryo lethality was observed for both double homozygous and AtCAP-E1-/-, AtCAP-E2+/- plants; arrest occurred at or before the globular stage and was associated with altered planes of cell division in both the suspensor and the embryo. Down regulation of both genes by antisense technology, as well as in AtCAP-E1+/-, AtCAP-E2-/- plants results in meristem disorganization and fasciation. Our data are consistent with the interpretation that threshold levels of SMC2 proteins are required for normal development and that AtCAP-E2 may have a higher affinity for its target than AtCAP-E1. CO- Development; Molecular Genetics (Biochemistry and Molecular Biophysics); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Developmental Biology -- Embryology-General and Descriptive (CC25502); Plant Physiology, Biochemistry and Biophysics -- Growth, Differentiation (CC51510) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis (Cruciferae) -- Landsberg erecta ecotype, embryo; Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- chromatin -- condensation; meristem -- disorganization, fasciation; meristematic cells; sister chromatid -- resolution DE- AtCAP-E1; AtCAP-E2; Structural Maintenance of Chromosomes 2 [SMC2]; Structural Maintenance of Chromosomes 4 [SMC4]; T-DNA; condensin complex DE- Arabidopsis AtCAP-E1 gene (Cruciferae); Arabidopsis AtCAP-E2 gene (Cruciferae); Arabidopsis condensin genes (Cruciferae): mutation; Arabidopsis titan gene (Cruciferae); yeast SMC2 gene [yeast Structural Maintenance of Chromosomes 2 gene] (Fungi); yeast SMC4 gene [yeast Structural Maintenance of Chromosomes 4 gene] (Fungi) DE- Arabidopsis genome project; cell division; chromosomal integrity -- maintenance; embryo lethality; embryogenesis; female gametogenesis; homologous chromosome segregation; male gametogenesis; meiosis; meristem organization DE- Fungi-Unspecified (BC15000); Cruciferae (BC25880) DT- Article LA- English SS- 0950-1991 CD- DEVPED ID- PREV200300378913 SI- 0950-1991(2003)130:14<3283:MIACGD>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #24 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0950-1991&date=2003& volume=130&issue=14&spage=3163&atitle=Stem%20cell%20homeostasis%20in%20the% 20Arabidopsis%20shoot%20meristem%20is%20regulated%20by%20intercellular%20mo vement%20of%20CLAVATA3%20and%20its%20sequestration%20by%20CLAVATA1.&aulast= Lenhard&auinit=Michael TI- Stem cell homeostasis in the Arabidopsis shoot meristem is regulated by intercellular movement of CLAVATA3 and its sequestration by CLAVATA1. AU- Lenhard, Michael; Laux, Thomas IN- Institut fuer Biologie III, Universitaet Freiburg, Schaenzlestrasse 1, D-79104, Freiburg, Germany Germany JN- Development (Cambridge) ; July 2003; v.130, no.14, p.3163-3173. JA- DEVELOPMENT (CAMB) AB- Stem cell maintenance in the Arabidopsis shoot meristem is regulated by communication between the apical stem cells and the underlying organizing centre. Expression of the homeobox gene WUSCHEL in the organizing centre induces stem cell identity in the overlying neighbours, which the express the CLAVATA3 gene whose activity in turn restricts the size of the WUSCHEL expression domain. We have analyzed how the stem cells and the organizing centre communicate, by studying the mode of action of CLAVATA3 protein. We provide direct evidence that CLAVATA3 protein functions as a mobile intercellular signal in the shoot apical meristem that spreads laterally from the stem cells and acts both on their lateral neighbours and on the stem cells themselves to repress WUSCHEL transcription. We also show that the spread and range of action of CLAVATA3 can be limited by binding to its receptor CLAVATA1, which offers an explanation for how CLAVATA3 is prevented from entering the organizing centre and repressing WUSCHEL transcription there. This regulated spread of a secreted signalling molecule enables the shoot meristem to permit the onset of cell differentiation in the periphery, but at the same time to maintain a stable niche for its stem cells in the center. CO- Development; Molecular Genetics (Biochemistry and Molecular Biophysics); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Developmental Biology -- Embryology-General and Descriptive (CC25502); Plant Physiology, Biochemistry and Biophysics -- Growth, Differentiation (CC51510) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Arabidopsis (Cruciferae) -- Landsberg erecta ecotype; Angiosperms; Dicots; Plants; Spermatophytes; Vascular Plants DE- apical stem cell; shoot meristem DE- CLAVATA1; CLAVATA3 -- intercellular movement, mobile intercellular signal DE- Arabidopsis CLV3 gene [ARabidopsis CLAVATA3 gene] (Cruciferae); Arabidopsis WUSCHEL gene (Cruciferae): homeobox gene, transcription repression DE- cell differentiation; stem cell homeostasis DE- Cruciferae (BC25880) DT- Article LA- English SS- 0950-1991 CD- DEVPED ID- PREV200300378903 SI- 0950-1991(2003)130:14<3163:SCHITA>2.0.TX;2- CZ- Copyright 2001 BIOSIS Record #25 FN BIOSIS at LANL AR- http://linkseeker.lanl.gov/stanford?genre=article&issn=0960-7412&date=2003& volume=35&issue=2&spage=193&atitle=Arabidopsis%20local%20resistance%20to%20 Botrytis%20cinerea%20involves%20salicylic%20acid%20and%20camalexin%20and%20 requires%20EDS4%20and%20PAD2,%20but%20not%20SID2,%20EDS5%20or%20PAD4.&aulas t=Ferrari&auinit=Simone TI- Arabidopsis local resistance to Botrytis cinerea involves salicylic acid and camalexin and requires EDS4 and PAD2, but not SID2, EDS5 or PAD4. AU- Ferrari, Simone; Plotnikova, Julia M.; De Lorenzo, Giulia; Ausubel, Frederick M. IN- Department of Genetics, Harvard Medical School, Massachusetts General Hospital, Boston, MA, 02114, USA USA; E-Mail: ausubel@molbio.mgh.harvard.edu JN- Plant Journal ; July 2003; v.35, no.2, p.193-205. JA- PLANT J AB- Salicylic acid (SA) is an important regulator of plant defense responses, and a variety of Arabidopsis mutants impaired in resistance against bacterial and fungal pathogens show defects in SA accumulation, perception, or signal transduction. Nevertheless, the role of SA-dependent defense responses against necrotrophic fungi is currently unclear. We determined the susceptibility of a set of previously identified Arabidopsis mutants impaired in defense responses to the necrotrophic fungal pathogen Botrytis cinerea. The rate of development of B. cinerea disease symptoms on primary infected leaves was affected by responses mediated by the genes EIN2, JAR1, EDS4, PAD2, and PAD3, but was largely independent of EDS5, SID2/ICS1, and PAD4. Furthermore, plants expressing a nahG transgene or treated with a phenylalanine ammonia lyase (PAL) inhibitor showed enhanced symptoms, suggesting that SA synthesized via PAL, and not via isochorismate synthase (ICS), mediates lesion development. In addition, the degree of lesion development did not correlate with defensin or PR1 expression, although it was partially dependent upon camalexin accumulation. Although npr1 mutant leaves were normally susceptible to B. cinerea infection, a double ein2 npr1 mutant was significantly more susceptible than ein2 plants, and exogenous application of SA decreased B. cinerea lesion size through an NPR1-dependent mechanism that could be mimicked by the cpr1 mutation. These data indicate that local resistance to B. cinerea requires ethylene-, jasmonate-, and SA-mediated signaling, that the SA affecting this resistance does not require ICS1 and is likely synthesized via PAL, and that camalexin limits lesion development. CO- Chemical Coordination and Homeostasis; Infection; Molecular Genetics (Biochemistry and Molecular Biophysics); Genetics and Cytogenetics -- General (CC03502); Genetics and Cytogenetics -- Plant (CC03504); Biochemical Studies -- General (CC10060); Physiology, General and Miscellaneous -- General (CC12002); Plant Physiology, Biochemistry and Biophysics -- Growth Substances (CC51514); Plant Physiology, Biochemistry and Biophysics -- Chemical Constituents (CC51522); Plant Physiology, Biochemistry and Biophysics -- General and Miscellaneous (CC51526); Phytopathology -- Parasitism and Resistance (CC54514) TX- Cruciferae -- Dicotyledones, Angiospermae, Spermatophyta, Plantae; Fungi Imperfecti or Deuteromycetes -- Fungi, Plantae; Arabidopsis thaliana (Cruciferae) -- host; Botrytis cinerea (Fungi Imperfecti or Deuteromycetes) -- pathogen; Angiosperms; Dicots; Fungi; Microorganisms; Nonvascular Plants; Plants; Spermatophytes; Vascular Plants DE- camalexin -- disease resistance; ethylene -- plant growth regulator, signaling; jasmonate -- plant growth regulator, signaling; salicylic acid -- disease resistance; 135531-86-1 -- CAMALEXIN; 74-85-1 -- ETHYLENE; 69-72-7 -- SALICYLIC ACID DE- Arabidopsis thaliana EDS4 gene (Cruciferae): disease-mediated response; Arabidopsis thaliana EDS5 gene (Cruciferae); Arabidopsis thaliana PAD2 gene (Cruciferae): disease-mediated response; Arabidopsis thaliana PAD4 gene (Cruciferae); Arabidopsis thaliana SID2 gene (Cruciferae) DE- plant defense responses -- local resistance DE- Fungi Imperfecti or Deuteromycetes (BC15500); Cruciferae (BC25880) DT- Article LA- English SS- 0960-7412 CD- PLJUED ID- PREV200300378692 SI- 0960-7412(2003)35:2<193:ALRTBC>2.0.TX;2- CZ- Copyright 2001 BIOSIS