OWN -   AGL
XX -   F832
XX -   F600
XX -   F200
AU -   He, P.
TI -   Activation of a COI1-dependent pathway in Arabidopsis by Pseudomonas syringae type III effectors and coronatine.
PG -   p. 589-602.
XX -   Includes references
AB -   Gram-negative bacteria use a variety of virulence factors including phytotoxins, exopolysaccharides, effectors secreted by the type III secretion system, and cell-wall-degrading enzymes to promote parasitism in plants. However, little is known about how these virulence factors alter plant cellular responses to promote disease. In this study, we show that virulent Pseudomonas syringae strains activate the transcription of an Arabidopsis ethylene response factor (ERF) gene, RAP2.6, in a coronatine insensitive 1 (COI1)-dependent manner. A highly sensitive RAP2.6 promoter-firefly luciferase (RAP2.6-LUC) reporter line was developed to monitor activities of various bacterial virulence genes. Analyses of P. syringae pv. tomato DC3000 mutants indicated that both type III secretion system and the phytotoxin coronatine are required for RAP2.6 induction. We show that at least five individual type III effectors, avirulence B (AvrB), AvrRpt2, AvrPphB, HopPtoK, and AvrPphEPto, contributed to RAP2.6 induction. Gene-for-gene recognition was not involved in RAP2.6 induction because plants lacking RPM1 and RPS2 responded normally to AvrB and AvrRpt2 in RAP2.6 expression. Interestingly, the role of coronatine in RAP2.6 induction can be partially substituted by the addition of avrB in DC3000, suggesting that AvrB may mimic coronatine. These results suggest that P. syringae type III effectors and coronatine act by augmenting a COI1-dependent pathway to promote parasitism.
OT -   Arabidopsis thaliana
OT -   transgenic plants
OT -   plant proteins
OT -   transcription factors
OT -   promoter regions
OT -   gene expression regulation
OT -   transcriptional activation
OT -   gene induction
OT -   jasmonic acid
OT -   signal transduction
OT -   ethylene
OT -   salicylic acid
OT -   resistance mechanisms
OT -   disease resistance
OT -   Pseudomonas syringae pv. tomato
OT -   Pseudomonas syringae pv. maculicola
OT -   Pseudomonas syringae pv. phaseolicola
OT -   Pseudomonas syringae pv. tabaci
OT -   Type III secretion system
OT -   bacterial toxins
OT -   virulence
OT -   pathogenicity
OT -   nucleotide sequences
OT -   coronatine insensitive 1 protein
OT -   coronatine
OT -   ethylene response factor
OT -   avirulence proteins
AU -   Chintamanani, S.
AU -   Chen, Z.
AU -   Zhu, L.
AU -   Kunkel, B.N.
AU -   Alfano, J.R.
AU -   Tang, X.
AU -   Zhou, J.M. 
TA -  Plant journal.  2004 Feb., v. 37, no. 4  1022630165 
IS -  0960-7412 
XX -  nnas 
XX -  jnl47643
CA -     QK710.P68  IND  RSC  2004/03/15  Non-US